Many of you have asked us how to make a solid deposit, we hope that this tutorial will answer your questions!
This technique is widely uses when crude sample is not soluble in the starting eluant conditions.

To make a solid deposit, the needed materials are:

  • A Dry-Load with a frit and a sample distributor (only for dry-loads F0040 to F0330)
  • The sample
  • A spatula
  • A funnel
  • Celite, silica or C18 in quantity 1:1 or 2:1 (celite:silica)
    NB: Celite doesn’t generate back pressure, doesn’t interact with compounds and improves the separation. Celite can be used in normal and reverse phase conditions.
  • A round bottom flask
  • A test-tube
  • Solvent to completely dissolve the sample


The different steps involved :

  1. Put the sample in a round bottom flask.
  2. Add solvent to dissolve the entire sample.
  3. Rinse the vial of the sample with solvent to recover a maximum of product.
  4. Put the celite in the round bottom flask
  5. Rinse the funnel with solvent
  6. Put the Solid deposit in the Dry-Load
  7. Then add celite to the top of the Dry-Load
  8. Slightly tamp down the celite and complete the Dry-Load again
  9. Put the top frit (and the sample distributor, depending on the Dry-Load’s size)
  10. Before closing the Dry-Load, check if the seal is correctly positioned
    To properly tighten the cap, you must first reach the abutment and tighten firmly of 1/6 of a turn


Know more:

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