Save time by using puriFlash Monolith column!

Due to their particular structure, the puriFLash® Monolith columns provide a great help for all the points on which a balance must be implemented.

The purification must be as fast as possible so as not to slow down the production process, which is why puriFlash® Monolith columns are essential for all purifications. They will save time for this important step, up to 80% less time.

 

What is a puriFlash® monolith column?

Interchim® Peptides monolith column is a pre-packed column with the novel silica gel for reversed-phase liquid chromatography that will permit high-speed processing only with a medium to low back pressure.

 

How puriFlash® monolith columns can provide best results?

In the pores of this monolith, there are two structures (Macro and micro pores), which allow a faster and deeper diffusion of the solvent inside the particles. That conduces to a more effective purification, especially of macromolecules such as peptides, with an extremely low pressure.

This structure makes it possible to work at flow rates 4 to 5 times greater than the optimum flow rate, and with a loss of resolution much less than conventional silica.

 

1. Less pressure

Save time for purification by increasing the flow-rate for your methods.
Loss of resolution will be low.

Application :

Dual pore	Conventional silica 15µm
Column F0025 @ 15mL/min	Column F0025 @ 15mL/min
2 x Column F0025 @ 15mL/min	2 x Column F0025 @ 15mL/min
2 x Column F0025 @ 60mL/min	High pressure even at 2 times optimum flow-rate
Samples: 1- GLY-TYR 238 2- VAL-TYR-VAL 380 3- Met-Enkephalin 574 4- Angiotensin 1 000 5- Cytochrome c from bovine heart 11749 Inj. 0.15mL	Gradient: water/ACN + 0.1%TFA 5 to 60% en ACN 15mL/min in 33.75min 15mL/min in 66.5min (2 coluns stacked) 60mL/min in 16.5min (2 coluns stacked) Detection : 215nm

 

2. Improve resolution of Flash

Comparison with conventional columns:

Riboflavin	Allura red AC	Cyanocobalamin

Normale Phase

DualPore	B 20 µm	Y 25 µm	G 20 µm	I 15 µm

Sample: Allura red AC, brilliant blue FCF, cyanocobalamin and rhodamine B, Eluant: hexane : ethanol = 70 : 30

Reversed Phase

DualPore	B 20 µm	Y 40 µm	Y 25 µm	G 20 µm	I 15 µm

Sample: Allura red AC, Fast green FCF, riboflavin and rhodamine B, Eluant: acetonitrile : water = 15 : 85

Compared to columns filled with a conventional silica, it can be seen that columns filled with a monolithic phase have a smaller sample diffusion band.

It becomes easier to purify polar compounds. Indeed, the weak broadening of the compound bands allows a better separation for close retention time compounds.

β-carotene	Brilliant Blue FCF	Rhodamine B

DualPore Flash	B 20 µm	I 15 µm

Sample: β-carotene, brilliant blue FCF, and rhodamine B, Hexane : ethanol = 7:3

Application for natural products

Purification of carotenoids

SIL : Spirilloxanthin extracted from Photosynthetic bacteria		ODS: lycopene extracted from tomato paste

To summarize the advantages of choosing Monolith puriFlash® columns :

• Low pressure. (It is possible to use solvents that generally generate high pressure)
• Increase productivity by using higher flow rates during purifications.
• Low diffusion of the sample in the column, allowing to separate compounds of close retention time.