Due to their particular structure, the puriFLash® Monolith columns provide a great help for all the points on which a balance must be implemented.

The purification must be as fast as possible so as not to slow down the production process, which is why puriFlash® Monolith columns are essential for all purifications. They will save time for this important step, up to 80% less time.

 

What is a puriFlash® monolith column?

Interchim® Peptides monolith column is a pre-packed column with the novel silica gel for reversed-phase liquid chromatography that will permit high-speed processing only with a medium to low back pressure.

 

How puriFlash® monolith columns can provide best results?

In the pores of this monolith, there are two structures (Macro and micro pores), which allow a faster and deeper diffusion of the solvent inside the particles. That conduces to a more effective purification, especially of macromolecules such as peptides, with an extremely low pressure.

This structure makes it possible to work at flow rates 4 to 5 times greater than the optimum flow rate, and with a loss of resolution much less than conventional silica.

 

1. Less pressure

Save time for purification by increasing the flow-rate for your methods.
Loss of resolution will be low.

Application :

Dual pore

Conventional silica 15µm

Column F0025 @ 15mL/min

Application Silice conventionnelle 15µm colonnes puriFlash Monotith

Column F0025 @ 15mL/min

Flèche Flèche
Application Dual pore colonnes puriFlash Monotith

2 x Column F0025 @ 15mL/min

Application Silice conventionnelle 15µm colonnes puriFlash Monotith

2 x Column F0025 @ 15mL/min

Flèche Flèche

Application Dual pore colonnes puriFlash Monotith

2 x Column F0025 @ 60mL/min

High pressure even at 2 times
optimum flow-rate

Samples:
1- GLY-TYR 238
2- VAL-TYR-VAL 380
3- Met-Enkephalin 574
4- Angiotensin 1 000
5- Cytochrome c from bovine heart 11749
Inj. 0.15mL
Gradient:
water/ACN + 0.1%TFA
5 to 60% en ACN 15mL/min in 33.75min
15mL/min in 66.5min (2 coluns stacked)
60mL/min in 16.5min (2 coluns stacked)
Detection :
215nm

 

2. Improve resolution of Flash

Comparison with conventional columns:

Riboflavin Allura red AC Cyanocobalamin
Riboflavin Allura Red AC Cyanocobalamin

Normale Phase

Riboflavin Colonne Phase normale B 20µm Colonne Phase normale Y 25µm Colonne Phase normale G 20um Colonne Phase normale I 15µm
DualPore B 20 µm Y 25 µm G 20 µm I 15 µm

Sample: Allura red AC, brilliant blue FCF, cyanocobalamin and rhodamine B, Eluant: hexane : ethanol = 70 : 30

Reversed Phase

Colonne Phase inverse DualPore Colonne Phase inverse B 20um Colonne Phase inverse Y 40µm Colonne Phase inverse Y 25µm Colonne Phase inverse G 20µm Colonne Phase inverse I 15µm
DualPore B 20 µm Y 40 µm Y 25 µm G 20 µm I 15 µm

Sample: Allura red AC, Fast green FCF, riboflavin and rhodamine B, Eluant: acetonitrile : water = 15 : 85

Compared to columns filled with a conventional silica, it can be seen that columns filled with a monolithic phase have a smaller sample diffusion band.

It becomes easier to purify polar compounds. Indeed, the weak broadening of the compound bands allows a better separation for close retention time compounds.

β-carotene Brilliant Blue FCF Rhodamine B
Beta carotene Brilliant Blue FCF Rhodamine B
DualPore Flash B 20 µm I 15 µm
Colonne DualPore FLASH Colonne B 20 µm Colonne I 15 µm
Flèche Flèche Flèche
Colonne Phase inverse B 20um Colonne B 20 µm Colonne I 15 µm

Sample: β-carotene, brilliant blue FCF, and rhodamine B, Hexane : ethanol = 7:3

Application for natural products

Purification of carotenoids

SIL : Spirilloxanthin extracted from Photosynthetic bacteria SIL : Spirilloxanthin extracted from Photosynthetic bacteria ODS: lycopene extracted from tomato paste
SIL : Spirilloxanthin extracted from Photosynthetic bacteria ODS: lycopene extracted from tomato paste

To summarize the advantages of choosing Monolith puriFlash® columns :

  • Low pressure. (It is possible to use solvents that generally generate high pressure)
  • Increase productivity by using higher flow rates during purifications.
  • Low diffusion of the sample in the column, allowing to separate compounds of close retention time.

 

Know more: