Analysis & purification of proteins: HIC, the separation of molecules according to their hydrophobicity
HIC is also known as “Salting out”. The separation is done following the hydrophobicity of the...
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Tips & Tricks
Analysis & purification of proteins: SEC (Size Exclusion Chromatography), the separation of molecules according to their sizes
To follow our set of articles speaking about proteins purification technics, today we are focusing...
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Protein purification technics : Affinity
Continuation of our series of articles on protein purification techniques : Last February we...
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Oh no! Not another boring webinar on column lifetime from Agilent?
If you are looking to refresh your HPLC skills; gain practical knowledge on longer lasting LC...
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TLC Fundamentals – Stationary & mobile phase choice (part 4)
Stationary phase choice: The choice of sorbent depends on the nature of the compounds to purify...
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TLC Fundamentals – How to make a successful TLC plate (part 3)
1. Deposit line & Solvent front Plot the deposit line with a pencil at 3mm above the mobile...
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TLC Fundamentals – Operational modes (part 2)
Different operational modes are commonly used to make a TLC plate => Unsaturated, Saturated,...
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TLC Fundamentals – Basic principles (part 1)
TLC, compared to liquid chromatography on column, shows differences: The mobile phase discovers...
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Purification of a sample: Solubility (part 2)
Solubility Purpose: understand how to choose the solvent that completely solubilizes a sample In a...
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Purification of a sample: Polarity (part 1)
This chapter was conceived and written in collaboration and under the supervision of Professor...
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