Three fast-spreading new variants of SARS-CoV-2 virus have emerged around the world: the U.K. variant B.1.1.7, the Brazil variant P.1, and the South Africa variant B.1.351

3 nouveaux variants SARS-CoV-2

Popular mutations in spike protein


These 3 lines carry the N501Y mutation and seem to have appeared independently of each other. They belong to the “mother” line B.1, resulting from the D614G mutation which appeared shortly after the start of the epidemic in Wuhan. These convergent mutations, specifically, the N501Y and E484K (shared between B.1.351 and P.1) are of high interest because they may be the cause of the increased transmissibility.

The mutations in these strains also occur in the nucleocapsid protein, which is commonly used as the biomarker in rapid antigen tests. It’s critical to assess whether the current commercial antigen tests can detect the mutated N proteins with the same sensitivity and specificity as their Wild Type counterpart. These proteins can serve also as targets of neutralizing antibodies under development.

SARS-CoV-2 S protein, His Tag, Super stable trimer

SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified, #SPN-C52H9)


A large panel of recombinant RBD/Spike and nucleocapsid protein variants carrying the aforementioned mutations is available as below:

Mutations of Variant B.1.1.7 in UK

Mutations of Variant B.1.351 in South Africa


Mutations of Variant P.1 in Brazil


Mutations of Variant Mink in Denmark

High Frequency Mutation of Nucleocapsid


Conserved Domain of Nucleocapsid from SARS-CoV-2


To go further

1. Detection of the new variants of SARS-CoV-2 (England, Brazil, and South Africa) with Norgen COVID19 TaqMan Detection RT-PCR kits

P/N Description Quantity
DXTM67100 2019-nCoV TaqMan RT-PCR Kit Dx 50 réactions
DxTM67120 2019-nCoV TaqMan RT-PCR Kit Dx 500 réactions
DxTM67200 COVID-19 TaqMan RT-PCR Kit (E/RdRP genes) Dx 500 réactions
TM67100 2019-nCoV TaqMan RT-PCR Kit 50 réactions
TM67120 2019-nCoV TaqMan RT-PCR Kit 500 réactions
TM67240 COVID-19 TaqMan RT-PCR Kit (E/RdRP genes) 40 réactions


2. Production of variant

P/N Description Quantity
VG40589-UT SARS-CoV-2 (2019-nCoV) Spike ORF mammalian expression plasmid 1 unité
15071 Muta-Direct™ Site-Directed Mutagenesis Kit 15 reactions
FP318 LipoFectMax™ 3000 Transfection Reagent 750µL
30081-T Firefly & Renilla Luciferase Single Tube Assay Kit 50 assays


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