Three fast-spreading new variants of SARS-CoV-2 virus have emerged around the world: the U.K. variant B.1.1.7, the Brazil variant P.1, and the South Africa variant B.1.351
Popular mutations in spike protein
These 3 lines carry the N501Y mutation and seem to have appeared independently of each other. They belong to the “mother” line B.1, resulting from the D614G mutation which appeared shortly after the start of the epidemic in Wuhan. These convergent mutations, specifically, the N501Y and E484K (shared between B.1.351 and P.1) are of high interest because they may be the cause of the increased transmissibility.
The mutations in these strains also occur in the nucleocapsid protein, which is commonly used as the biomarker in rapid antigen tests. It’s critical to assess whether the current commercial antigen tests can detect the mutated N proteins with the same sensitivity and specificity as their Wild Type counterpart. These proteins can serve also as targets of neutralizing antibodies under development.
SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified, #SPN-C52H9)
A large panel of recombinant RBD/Spike and nucleocapsid protein variants carrying the aforementioned mutations is available as below:
Mutations of Variant B.1.1.7 in UK
Mutations of Variant B.1.351 in South Africa
Mutations of Variant P.1 in Brazil
Mutations of Variant Mink in Denmark
High Frequency Mutation of Nucleocapsid
Conserved Domain of Nucleocapsid from SARS-CoV-2
To go further
1. Detection of the new variants of SARS-CoV-2 (England, Brazil, and South Africa) with Norgen COVID19 TaqMan Detection RT-PCR kits
| P/N | Description | Quantity |
| DXTM67100 | 2019-nCoV TaqMan RT-PCR Kit Dx | 50 réactions |
| DxTM67120 | 2019-nCoV TaqMan RT-PCR Kit Dx | 500 réactions |
| DxTM67200 | COVID-19 TaqMan RT-PCR Kit (E/RdRP genes) Dx | 500 réactions |
| TM67100 | 2019-nCoV TaqMan RT-PCR Kit | 50 réactions |
| TM67120 | 2019-nCoV TaqMan RT-PCR Kit | 500 réactions |
| TM67240 | COVID-19 TaqMan RT-PCR Kit (E/RdRP genes) | 40 réactions |
2. Production of variant
| P/N | Description | Quantity |
| VG40589-UT | SARS-CoV-2 (2019-nCoV) Spike ORF mammalian expression plasmid | 1 unité |
| 15071 | Muta-Direct™ Site-Directed Mutagenesis Kit | 15 reactions |
| FP318 | LipoFectMax™ 3000 Transfection Reagent | 750µL |
| 30081-T | Firefly & Renilla Luciferase Single Tube Assay Kit | 50 assays |
Know more:
- Download the Critical Reagents for SARS-CoV-2 Research brochure
- Do not hesitate to consult us to benefit from our expertise
- Consult our BioSciences catalog
