Troubleshooting His-Tagged Protein Binding to Ni-NTA Columns
Poly-histidine (His)-tagged proteins are commonly used in recombinant protein expression and...
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Purification
Webinar replay: Continuous purification from flow chemistry systems with in-line flash chromatography
Watch the replay of our webinar with Christopher Thomson, Researcher at Heriot-Watt University, as...
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Quick and efficient purification and identification of large peptides with puriFlash® MS
During various discussions about the puriFlash® MS, the maximum mass limit of this detector (2000...
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Lysis Buffers for Protein Extraction
The proteomic analysis starts with lysis, followed by purification. Lysis buffers include...
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Biotinylated proteins for quantification, detection and purification
The specific interaction between biotin and avidin (streptavidin) makes it an excellent tool to...
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Intelligent flash purification using TLC and MASS spectrometry
Introduction The compound synthesis, purification and confirmation process often presents...
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Peptides purification: puriFlash® 5.250P – puriFlash® expression coupling
This year has been very special for everyone, in order to adapt, Interchim® has set up a...
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Purification of Natural products : Boosting the antifungal drug discovery by halogenating plant extracts to obtain bioactive “unnatural” natural products
1. Introduction It is estimated that 20 percent of all pharmaceutical small molecule drugs are...
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Analysis & purification of proteins: HIC, the separation of molecules according to their hydrophobicity
HIC is also known as “Salting out”. The separation is done following the hydrophobicity of the...
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Analysis & purification of proteins: SEC (Size Exclusion Chromatography), the separation of molecules according to their sizes
To follow our set of articles speaking about proteins purification technics, today we are focusing...
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