Since the end of December 2019, a new coronavirus (named nCov-2019) from Wuhan is spreading rapidly in China and the rest of the world. Preventing this spread is achieved by quarantining and screening potentially infected people.

 

Identification of the viral strain of this coronavirus

Chinese scientists have now sequenced the viral genome and made its sequence available to assist the scientific community with rapid vaccine development .

This coronavirus appears to be a group 2B coronaviruses (only the alpha and beta coronaviruses have the capability of infecting humans) and shares 70% similarity to SARS-related bat coronavirus

Coronavirus nCov-2019

 

The main coronavirus proteins of nCoV-2019

The nucleocapsid protein (N protein)

The nucleocapsid protein (N protein) is the most abundant protein in coronaviruses. Highly immunogenic, this phosphoprotein is very well conserved across coronavirus families. It is often used as a marker in diagnostic tests. The strong homology (nearly 90%) between nCoV-2019 and SARS nucleocapsid (N) proteins would allow the use of SARS N protein specific antibodies for nCoV-2019 diagnostic tests.

 

The spike protein (S protein)

The spike protein (S protein) mediates receptor binding and fusion of viral and cell membranes. The spike protein contains two subunits, S1 and S2. S1 contains a receptor binding domain (RBD), which is responsible for recognition and binding to the cell surface receptor. The S2 subunit is the “stem” of the structure, which contains other basic elements necessary for membrane fusion. The spike protein is the common target of neutralizing antibodies and vaccines. Proteine spike

InterchimĀ®, through SinoBiological, is now able to offer you cDNA clones, proteins specific to nCOV-2019. Primary antibodies specific to nCov-2019 will soon be added to the existing offer.

We provide recombinant proteins include the complete S protein (ECD), S1 subunit, S2 subunit, RBD S1 and N protein. All corresponding references are listed in the following table:

Proteins

Reference Description Specificity Host Tag
40589-V08B1 2019-nCoV Spike Protein (S1+S2 ECD, His tag) 2019-nCoV Baculovirus-Insect Cells C-His
40591-V05H1 2019-nCoV Spike Protein (S1 Subunit, mFc Tag) 2019-nCoV Human Cells C-Fc
40592-V05H 2019-nCoV Spike Protein (RBD, Fc Tag) 2019-nCoV Human Cells C-Fc
40588-V08B 2019-nCoV Nucleocapsid Protein (His tag) 2019-nCoV Baculovirus-Insect Cells C-His
40591-V08B1 2019-nCoV Spike Protein (S1 Subunit, His tag) 2019-nCoV Baculovirus-Insect Cells C-His
40591-V08H 2019-nCoV Spike Protein S1 (His Tag) 2019-nCoV Human Cells C-His
40590-V08B 2019-nCoV Spike Protein (S2 ECD, His tag) 2019-nCoV Baculovirus-Insect Cells C-His
40591-V02H 2019-nCoV Spike Protein S1 (Fc Tag) 2019-nCoV Human Cells C-Fc

 

Plasmids

Reference Description Specificity
VG40588-UT 2019-nCoV Nucleoprotein / NP ORF mammalian expression plasmid
(Codon Optimized)
2019-nCoV
VG40589-UT 2019-nCoV Spike ORF mammalian expression plasmid (Codon Optimized) 2019-nCoV
VG40590-UT 2019-nCoV Spike(S2) ORF mammalian expression plasmid (Codon Optimized) 2019-nCoV
VG40591-UT 2019-nCoV Spike(S1) ORF mammalian expression plasmid (Codon Optimized) 2019-nCoV
VG40592-UT 2019-nCoV Spike(RBD) ORF mammalian expression plasmid (Codon Optimized) 2019-nCoV

 

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